Scientific Objectives and Experimental Design

Phenotyping

The TERRA-REF project is phenotyping the same genotypes of sorghum at multiple locations

Genotyping

Whole genome resequencing is being carried out on ~400 sorghum accessions to understand the landscape of genetic variation in the selected germplasm and enable high-resolution mapping of bioenergy traits with genome wide association studies (GWAS). Additionally, ~200 sorghum recombinant inbred lines (RILs) will be characterized with ~400,000 genetic markers using genotyping-by-sequencing (Morris et al., 2013) for trait dissection in the RIL population and testcross hybrids of the RIL population.

Maricopa Agricultural Center (MAC), Arizona

Three hundred thirty one lines were planted in 2016. Plantings occurred both under and west of the gantry system.

Automated controlled-environment phenotyping, Missouri

Kansas State University

  • PhenoTractor - Coming 2017

  • UAV - Coming 2017

  • Manually collected field data - Coming 2017

HudsonAlpha - Genomics

  • Coming 2017