Experimental Design

Overview

Phenotyping

The TERRA-REF project is collecting phenotype data from 852 sorghum genotypes grown in three locations:

Data source

MAC

KSU

Danforth

Scanalyzer Field System

X

Scanalyzer 3D System

X

Phenotractor

X

X

UAV (drone)

X

X

Manual field data

X

X

Genotyping

Whole genome resequencing is being carried out on ~400 sorghum accessions to understand the landscape of genetic variation in the selected germplasm and enable high-resolution mapping of bioenergy traits with genome wide association studies (GWAS). Additionally, ~200 sorghum recombinant inbred lines (RILs) will be characterized with ~400,000 genetic markers using genotyping-by-sequencing (Morris et al., 2013) for trait dissection in the RIL population and testcross hybrids of the RIL population.