Genomics

​Whole genome resequencing was carried out on ~400 sorghum accessions to understand the landscape of genetic variation in the selected germplasm and enable high-resolution mapping of bioenergy traits with genome wide association studies (GWAS). Additionally, ~200 sorghum recombinant inbred lines (RILs) were characterized with ~400,000 genetic markers using genotyping-by-sequencing (Morris et al., 2013) for trait dissection in the RIL population and testcross hybrids of the RIL population.
Whole-genome resequencing
Experimental Design:
384 BAP samples were sequenced to an average depth of ~25x.
​List of BAP accessions in BETYdb​
Shotgun sequencing (127-bp paired-end) was done using an Illumina X10 instrument at the HudsonAlpha Institute for Biotechnology.
Variant calling was done using a computational pipeline at the Danforth Center. 
Data were aligned against the BTx623 reference genotype
NCBI https://www.ncbi.nlm.nih.gov/assembly/GCF_000003195.3/​
Phytozome https://phytozome.jgi.doe.gov/pz/portal.html#!info?alias=Org_Sbicolor​
Genotyping-by-sequencing
Experimental Design:
​768 RIL samples were sequenced using a GBS approach.
See Also
​Genomics Protocols​
​Genomics Data Availability​
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Controlled Environment Phenotyping

Data

Last modified 3yr ago

Genomics

Whole-genome resequencing

Genotyping-by-sequencing

See Also

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