Whole genome resequencing was carried out on ~400 sorghum accessions to understand the landscape of genetic variation in the selected germplasm and enable high-resolution mapping of bioenergy traits with genome wide association studies (GWAS). Additionally, ~200 sorghum recombinant inbred lines (RILs) were characterized with ~400,000 genetic markers using genotyping-by-sequencing (Morris et al., 2013) for trait dissection in the RIL population and testcross hybrids of the RIL population.
384 BAP samples were sequenced to an average depth of ~25x.
Shotgun sequencing (127-bp paired-end) was done using an Illumina X10 instrument at the HudsonAlpha Institute for Biotechnology.
Variant calling was done using a computational pipeline at the Danforth Center.
Data were aligned against the BTx623 reference genotype
768 RIL samples were sequenced using a GBS approach.