Controlled Environment Protocols
Abstract
Automated VIS and NIR imaging in a controlled growth environment
Materials
ProMix BRK20 + 14-14-14 Osmocote pots
pre-filled by Hummert Sorghum seed
Equipment
Conviron Growth House
LemnaTec moving field conveyor belt system
Scanalyzer 3D platform
Procedures
Planting
Plant directly into phenotyping pots
Chamber Conditions
Pre-growth (11 days) and Phenotying (11 days)
14 hour photoperiod
32oC day/22oC night temperature
60% relative humidity
700 umol/m2/s light
Watering Conditions
Prior to phenotyping, plants watered daily
The first night after loading, plants watered 1× by treatment group to 100% field capacity (fc)
Days 2 – 12, plants watered 2× daily by treatment group (100% or 30% FC) to target weight
Automation
Left shift lane rotation within each GH, during overnight watering jobs
VIS (TV and 2 x SV), NIR (TV and 2 x SV) imaging daily
Recipes
Field capacity = 200% GWC (200 g water/100 g soil), based upon extensive GWC testing done by Skyler Mitchell
Target weight (fc) = [(water weight at % fc) + [(average weight of carrier/saucer) + (dry soil weight) + (pot weight)]
Water weight at 100% fc = dry soil weight * (%GWC/100)
Water weight at 30% fc = water weight at 100% fc * 0.30
References
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