Controlled Environment Protocols
Authors: Mockler Lab
Automated VIS and NIR imaging in a controlled growth environment
- ProMix BRK20 + 14-14-14 Osmocote pots
- pre-filled by Hummert Sorghum seed
- Conviron Growth House
- LemnaTec moving field conveyor belt system
- Scanalyzer 3D platform
Planting
- Plant directly into phenotyping pots
Chamber Conditions
Pre-growth (11 days) and Phenotying (11 days)
- 14 hour photoperiod
- 32oC day/22oC night temperature
- 60% relative humidity
- 700 umol/m2/s light
Watering Conditions
- Prior to phenotyping, plants watered daily
- The first night after loading, plants watered 1× by treatment group to 100% field capacity (fc)
- Days 2 – 12, plants watered 2× daily by treatment group (100% or 30% FC) to target weight
Automation
- Left shift lane rotation within each GH, during overnight watering jobs
- VIS (TV and 2 x SV), NIR (TV and 2 x SV) imaging daily
- Field capacity = 200% GWC (200 g water/100 g soil), based upon extensive GWC testing done by Skyler Mitchell
- Target weight (fc) = [(water weight at % fc) + [(average weight of carrier/saucer) + (dry soil weight) + (pot weight)]
- Water weight at 100% fc = dry soil weight * (%GWC/100)
- Water weight at 30% fc = water weight at 100% fc * 0.30
Last modified 3yr ago