Controlled Environment Protocols
1
Authors: Mockler Lab
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Abstract

Automated VIS and NIR imaging in a controlled growth environment

Materials

    ProMix BRK20 + 14-14-14 Osmocote pots
    pre-filled by Hummert Sorghum seed

Equipment

    Conviron Growth House
    LemnaTec moving field conveyor belt system
    Scanalyzer 3D platform

Procedures

Planting
    Plant directly into phenotyping pots 
Chamber Conditions
Pre-growth (11 days) and Phenotying (11 days)
    14 hour photoperiod
    32oC day/22oC night temperature
    60% relative humidity
    700 umol/m2/s light
Watering Conditions
    Prior to phenotyping, plants watered daily
    The first night after loading, plants watered 1× by treatment group to 100% field capacity (fc)
    Days 2 – 12, plants watered 2× daily by treatment group (100% or 30% FC) to target weight
Automation
    Left shift lane rotation within each GH, during overnight watering jobs
    VIS (TV and 2 x SV), NIR (TV and 2 x SV) imaging daily

Recipes

    Field capacity = 200% GWC (200 g water/100 g soil), based upon extensive GWC testing done by Skyler Mitchell
    Target weight (fc) = [(water weight at % fc) + [(average weight of carrier/saucer) + (dry soil weight) + (pot weight)]
    Water weight at 100% fc = dry soil weight * (%GWC/100)
    Water weight at 30% fc = water weight at 100% fc * 0.30

References

Last modified 1yr ago