Controlled Environment Protocols

Authors: Mockler Lab

Abstract

Automated VIS and NIR imaging in a controlled growth environment

Materials

  • ProMix BRK20 + 14-14-14 Osmocote pots

  • pre-filled by Hummert Sorghum seed

Equipment

  • Conviron Growth House

  • LemnaTec moving field conveyor belt system

  • Scanalyzer 3D platform

Procedures

Planting

  • Plant directly into phenotyping pots 

Chamber Conditions

Pre-growth (11 days) and Phenotying (11 days)

  • 14 hour photoperiod

  • 32oC day/22oC night temperature

  • 60% relative humidity

  • 700 umol/m2/s light

Watering Conditions

  • Prior to phenotyping, plants watered daily

  • The first night after loading, plants watered 1× by treatment group to 100% field capacity (fc)

  • Days 2 – 12, plants watered 2× daily by treatment group (100% or 30% FC) to target weight

Automation

  • Left shift lane rotation within each GH, during overnight watering jobs

  • VIS (TV and 2 x SV), NIR (TV and 2 x SV) imaging daily

Recipes

  • Field capacity = 200% GWC (200 g water/100 g soil), based upon extensive GWC testing done by Skyler Mitchell

  • Target weight (fc) = [(water weight at % fc) + [(average weight of carrier/saucer) + (dry soil weight) + (pot weight)]

  • Water weight at 100% fc = dry soil weight * (%GWC/100)

  • Water weight at 30% fc = water weight at 100% fc * 0.30

References