Experimental Design MAC

Season 1 sorghum (April - July 2016) Season 2 sorghum (August - November 2016) Durum wheat (January 2017 -

Season 1 soghum (April - July 2016)

Three hundred thirty one lines were planted in Season 1.

Planting maps

• Under the scanner system

• West the scanner system

Planting Design

Under scanner system

Experiment	Reps	Treatments	Experimental design
BAP	3	30 lines (12 PS, 12 sweet, 6 grain)	RCB with sorghum types nested in groups
Night illumination	3	5 illumination levels x 2 PS lines (with check line separating illumination levels)	RCB
Row #	3	6 adjacent plot scenarios: 3 lines (forage, sweet, PS) x 2 sides (east or west)	RCB but not balanced with all treatments in all reps
Biomass	3	5 sampling times x 3 lines (forage, sweet, PS)	RCB with sampling time as a repeated measure
Density	3	3 densities (5, 15, 30 cm) x 3 lines (forage, sweet, PS)	RCB
RILs	3	130 RILs plus 10 repeats of a single line/rep	Incomplete Block (row-column alpha lattice design)
Uniformity	17	2 lines (forage, PS)	None - Same line planted in single range

West of scanner system

Experiment	Reps	Treatments	Experimental design
BAP	1	30 lines (12 PS, 12 sweet, 6 grain)	None - single rep planted for observation
RILs	3	60 RILs	Incomplete Block (row-column alpha lattice design)

Automated Phenotpying

The Lemnatec Scanalyzer Field Gantry System is the largest field crop analytics robot in the world. This high-throughput phenotyping field-scanning robot has a 30-ton steel gantry that autonomously moves along two 200-meter steel rails while continuously imaging the crops growing below it with a diverse array of cameras and sensors.

Twelve sensors are attached to the gantry system. Detailed information for each sensor including name, variable measured, and field of view are available here. The planned sensor missions and their objectives for 2016 are available here.

Manually Collected Field Data

emergence vigor emergence final stand counts plant heights node and tiller counts on marked plants phenology growth stage data leaf desiccation ratings radiation interception managements Incomplete harvest yield data

Season 2 soghum (August - November 2016)

One hundred and seventy-six lines were planted in Season 2.

Planting map

Under the scanner system

Planting Design

Under scanner system - same as season 1

Automated Phenotpying

same as season 1

Manually Collected Field Data

plant heights managements emergence vigor emergence final stand counts node and tiller counts on marked plants leaf length and width on marked plants, one date

Durum wheat

Experimental design

Automatically and manually collected field data plan