# Controlled Environment Protocols

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Authors: Mockler Lab
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## Abstract

Automated VIS and NIR imaging in a controlled growth environment

## Materials

ProMix BRK20 + 14-14-14 Osmocote pots; pre-filled by Hummert Sorghum seed

## Equipment

* Conviron Growth House
* LemnaTec moving field conveyor belt system 
* Scanalyzer 3D platform

## Procedures

**Planting**

* Plant directly into phenotyping pots 

**Chamber Conditions**

Pre-growth (11 days) and Phenotying (11 days)

* 14 hour photoperiod
* 32oC day/22oC night temperature
* 60% relative humidity
* 700 umol/m2/s light

**Watering Conditions**

* Prior to phenotyping, plants watered daily
* The first night after loading, plants watered 1× by treatment group to 100% field capacity (fc)
* Days 2 – 12, plants watered 2× daily by treatment group (100% or 30% FC) to target weight

**Automation**

* Left shift lane rotation within each GH, during overnight watering jobs
* VIS (TV and 2 x SV), NIR (TV and 2 x SV) imaging daily

## Recipes

* **Field capacity** = 200% GWC (200 g water/100 g soil), based upon extensive GWC testing done by Skyler Mitchell
* **Target weight** (fc) = \[(water weight at % fc) + \[(average weight of carrier/saucer) + (dry soil weight) + (pot weight)]
* **Water weight** at 100% fc = dry soil weight \* (%GWC/100)
* **Water weight** at 30% fc = water weight at 100% fc \* 0.30

## References