# Experimental Design Genomics Genomic data includes whole-genome resequencing data from the HudsonAlpha Institute for Biotechnology, Alabama for 384 samples for accessions from the sorghum Bioenergy Association Panel (BAP) and genotyping-by-sequencing (GBS) data from Kansas State University for 768 samples from a population of sorghum recombinant inbred lines (RIL). ## Whole-genome resequencing **Experimental Design:** * [384 BAP samples](https://github.com/terraref/documentation/tree/56f669dc870b3c3921bfc029914545574e70f8df/user/user/sorghum-lines-genomics.md) were sequenced to an average depth of \~25x. * Shotgun sequencing (127-bp paired-end) was done using an Illumina X10 instrument at the HudsonAlpha Institute for Biotechnology. * Variant calling was done using a [computational pipeline](https://github.com/terraref/documentation/tree/56f669dc870b3c3921bfc029914545574e70f8df/user/genomic-data-product.md) at the Danforth Center. * See the [Data Products](https://github.com/terraref/documentation/tree/56f669dc870b3c3921bfc029914545574e70f8df/user/products/genomics-data.md) page to get access to raw and derived data products. ## Genotyping-by-sequencing **Experimental Design:** * [768 RIL samples](https://github.com/terraref/documentation/tree/56f669dc870b3c3921bfc029914545574e70f8df/user/user/genotyping-by-sequencing.md) were sequenced using a GBS approach. --- # Agent Instructions: Querying This Documentation If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question. Perform an HTTP GET request on the current page URL with the `ask` query parameter: ``` GET https://docs.terraref.org/beta/scientific-objectives-and-experimental-design/experimental-design/experimental-design-genomics.md?ask= ``` The question should be specific, self-contained, and written in natural language. The response will contain a direct answer to the question and relevant excerpts and sources from the documentation. Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.