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  • Introduction
  • Data Sources
  • Software
  • Scientific Objectives and Experimental Design
    • Protocols
      • Controlled Environment Protocols
      • Manual Field Data Protocols
      • Phenotractor Protocols
      • Sensor Calibration
      • Template Protocol
      • UAV Protocols
    • Experimental Design
      • Experimental Design Danforth
        • Sorghum Lines Danforth
      • Experimental Design Genomics
        • Sorghum Lines Genomics Year 1
        • Sorghum Lines Genomics Year 1 (continued)
        • Sorghum Lines Genomics Year 2
      • Experimental Design MAC
  • User Manual
    • What Data is Available
    • Data Products
      • Environmental conditions
      • Fluorescence intensity imaging
      • Genomics data
      • Geospatial information
      • Hyperspectral imaging data
      • Infrared heat imaging data
      • Multispectral imaging data
      • Meteorological data
      • Phenotype data
      • Point Cloud Data
    • How to Access Data
      • Using Clowder (Sensor and Genoomics data)
      • Using Globus (Sensor and Genomics data)
      • Using BETYdb (trait data, experimental metadata)
        • Accessing BETYdb via ArcMap and other GIS software
      • Using CoGe (Genomics)
      • Using CyVerse (Genomics)
      • Using Analysis Workbench (all data)
    • Data Use Policy
    • Manuscripts and Authorship Guidelines
    • Release / reprocessing schedule
  • Technical Documentation
    • Data Standards
      • Existing Data Standards
      • Agronomic and Phenotype Data Standards
      • Genomic Data Standards
      • Sensor Data Standards
      • Data Standards Committee
    • Directory Structure
    • Data Storage
    • Data Transfer
    • Data Processing Pipeline
      • Geospatial Time Series Structure
    • Data Backup
    • Data Collection
    • Data Product Creation
      • Genomic Data
      • Hyperspectral Data
    • Quality Assurance and Quality Control
    • Systems Configuration
  • Developer Manual
    • Submitting data to Clowder
    • Submitting data to BETYdb
    • Submitting Data to CoGe
    • Developing Clowder Extractors
  • Tutorials
  • Appendix
    • Code of Conduct
    • Collaboration Tools
    • Glossary
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  1. Scientific Objectives and Experimental Design
  2. Protocols

Controlled Environment Protocols

Authors: Mockler Lab

Abstract

Automated VIS and NIR imaging in a controlled growth environment

Materials

ProMix BRK20 + 14-14-14 Osmocote pots; pre-filled by Hummert Sorghum seed

Equipment

  • Conviron Growth House

  • LemnaTec moving field conveyor belt system

  • Scanalyzer 3D platform

Procedures

Planting

  • Plant directly into phenotyping pots 

Chamber Conditions

Pre-growth (11 days) and Phenotying (11 days)

  • 14 hour photoperiod

  • 32oC day/22oC night temperature

  • 60% relative humidity

  • 700 umol/m2/s light

Watering Conditions

  • Prior to phenotyping, plants watered daily

  • The first night after loading, plants watered 1× by treatment group to 100% field capacity (fc)

  • Days 2 – 12, plants watered 2× daily by treatment group (100% or 30% FC) to target weight

Automation

  • Left shift lane rotation within each GH, during overnight watering jobs

  • VIS (TV and 2 x SV), NIR (TV and 2 x SV) imaging daily

Recipes

  • Field capacity = 200% GWC (200 g water/100 g soil), based upon extensive GWC testing done by Skyler Mitchell

  • Target weight (fc) = [(water weight at % fc) + [(average weight of carrier/saucer) + (dry soil weight) + (pot weight)]

  • Water weight at 100% fc = dry soil weight * (%GWC/100)

  • Water weight at 30% fc = water weight at 100% fc * 0.30

References

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Last updated 7 years ago