Experimental Design

Overview

Phenotyping

The TERRA-REF project is collecting phenotype data from 852 sorghum genotypes grown in three locations:

• Maricopa Agricultural Center (MAC), Maricopa, Arizona

• Kansas State University (KSU), Ashland, KS

• Donald Danforth Plant Science Center, Missouri

Data source	MAC	KSU	Danforth
Scanalyzer Field System	X
Scanalyzer 3D System			X
Phenotractor	X	X
UAV (drone)	X	X
Manual field data	X	X

Genotyping

Whole genome resequencing is being carried out on ~400 sorghum accessions to understand the landscape of genetic variation in the selected germplasm and enable high-resolution mapping of bioenergy traits with genome wide association studies (GWAS). Additionally, ~200 sorghum recombinant inbred lines (RILs) will be characterized with ~400,000 genetic markers using genotyping-by-sequencing (Morris et al., 2013) for trait dissection in the RIL population and testcross hybrids of the RIL population.